16s rrna pyrosequencing Search Results


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( A ) Study 1. Macaques were immunized with live-attenuated S. dysenteriae 1 strain (CVD1256 or CVD1255) or mock-immunized with PBS on days 0 and 28, followed by wild-type S. dysenteriae 1 strain 1617 challenge on day 56. ( B ) Study 2. Macaques were immunized with CVD1256 or mock-immunized with PBS on days 0, 2, 4, and 7, followed by wild-type challenge with S. dysenteriae 1 strain 1617 on day 28. ( C ) Study 3. Macaques were challenged with wild-type S. dysenteriae 1 strain 1617. ( D ) Study 4. Macaques received no intervention. For all studies, samples were collected at labeled time points (days) for 16 S <t>rRNA</t> <t>pyrosequencing.</t> Immunization types occurred on indicated days (inset box).
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analysis of bacterial and archaeal 16s rrna genes  (Pyrosequencing Inc)
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( A ) Study 1. Macaques were immunized with live-attenuated S. dysenteriae 1 strain (CVD1256 or CVD1255) or mock-immunized with PBS on days 0 and 28, followed by wild-type S. dysenteriae 1 strain 1617 challenge on day 56. ( B ) Study 2. Macaques were immunized with CVD1256 or mock-immunized with PBS on days 0, 2, 4, and 7, followed by wild-type challenge with S. dysenteriae 1 strain 1617 on day 28. ( C ) Study 3. Macaques were challenged with wild-type S. dysenteriae 1 strain 1617. ( D ) Study 4. Macaques received no intervention. For all studies, samples were collected at labeled time points (days) for 16 S <t>rRNA</t> <t>pyrosequencing.</t> Immunization types occurred on indicated days (inset box).
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( A ) Study 1. Macaques were immunized with live-attenuated S. dysenteriae 1 strain (CVD1256 or CVD1255) or mock-immunized with PBS on days 0 and 28, followed by wild-type S. dysenteriae 1 strain 1617 challenge on day 56. ( B ) Study 2. Macaques were immunized with CVD1256 or mock-immunized with PBS on days 0, 2, 4, and 7, followed by wild-type challenge with S. dysenteriae 1 strain 1617 on day 28. ( C ) Study 3. Macaques were challenged with wild-type S. dysenteriae 1 strain 1617. ( D ) Study 4. Macaques received no intervention. For all studies, samples were collected at labeled time points (days) for 16 S <t>rRNA</t> <t>pyrosequencing.</t> Immunization types occurred on indicated days (inset box).
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( A ) Study 1. Macaques were immunized with live-attenuated S. dysenteriae 1 strain (CVD1256 or CVD1255) or mock-immunized with PBS on days 0 and 28, followed by wild-type S. dysenteriae 1 strain 1617 challenge on day 56. ( B ) Study 2. Macaques were immunized with CVD1256 or mock-immunized with PBS on days 0, 2, 4, and 7, followed by wild-type challenge with S. dysenteriae 1 strain 1617 on day 28. ( C ) Study 3. Macaques were challenged with wild-type S. dysenteriae 1 strain 1617. ( D ) Study 4. Macaques received no intervention. For all studies, samples were collected at labeled time points (days) for 16 S rRNA pyrosequencing. Immunization types occurred on indicated days (inset box).

Journal: PLoS ONE

Article Title: Differential Response of the Cynomolgus Macaque Gut Microbiota to Shigella Infection

doi: 10.1371/journal.pone.0064212

Figure Lengend Snippet: ( A ) Study 1. Macaques were immunized with live-attenuated S. dysenteriae 1 strain (CVD1256 or CVD1255) or mock-immunized with PBS on days 0 and 28, followed by wild-type S. dysenteriae 1 strain 1617 challenge on day 56. ( B ) Study 2. Macaques were immunized with CVD1256 or mock-immunized with PBS on days 0, 2, 4, and 7, followed by wild-type challenge with S. dysenteriae 1 strain 1617 on day 28. ( C ) Study 3. Macaques were challenged with wild-type S. dysenteriae 1 strain 1617. ( D ) Study 4. Macaques received no intervention. For all studies, samples were collected at labeled time points (days) for 16 S rRNA pyrosequencing. Immunization types occurred on indicated days (inset box).

Article Snippet: A total of 374 fecal samples were collected and the composition of the macaque intestinal microbiota was characterized using 16 S rRNA pyrosequencing of the V1–V2 region (Materials and Methods).

Techniques: Labeling